Here are some lab-ready, step-by-step protocols for duplex oligo ligation cloning and oligo phosphorylation and dephosphorylation!

A streamlined cloning method for small inserts and sequence repairs. Some cloning strategies require large DNA fragments or PCR amplification. Duplex oligo ligation cloning is a much simpler approach, perfect for repairing short sequences, inserting small elements, or fixing repetitive regions such as poly(A) tails . Let’s waddle through the process! 🐧 Step 1 – Identify the Region to Repair First, locate the region of the plasmid that needs to be repaired or modified. I